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appropriate fluorescent secondary antibodies  (Thermo Fisher)


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    Thermo Fisher appropriate fluorescent secondary antibodies
    Appropriate Fluorescent Secondary Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/appropriate+fluorescent+secondary+antibodies/pm39555723-246-20-24
    Average 90 stars, based on 1 article reviews
    appropriate fluorescent secondary antibodies - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: PPP2R3A Affects the Function and Mechanism of Heterogeneous Fibroblasts in Pulmonary Fibrosis
    Article Snippet: Then, the cells were washed 3 times with PBS, denatured with 2 N HCl/0.3% Triton X-100 at room temperature for 30 minutes, incubated with 0.1 M boric acid buffer (pH 8.0) for 10 minutes and blocked with goat serum at room temperature for 2 h. The cells were incubated with BrdU antibody (1:100; SC-32323, Santa Cruz) at 4°C overnight. .. After the PBS washes, the cells were incubated with appropriate uorescent secondary antibodies (Alexa Fluor, Thermo Fisher Scienti c) in the dark for 2 h. The cells were then washed 3 times with PBS and xed with a xative (Prolong Gold antifade reagent with DAPI; P36931, Life Technologies). .. The slides were imaged using a uorescence microscope (Olympus IX70, Olympus America, Inc., Center Valley, PA, USA).

    Staining:

    Article Title: Muscle fibroblasts and stem cells stimulate motor neurons in an age and exercise-dependent manner.
    Article Snippet: Samples were imaged with a 10×/0.30 NA objective and a 0.5× camera (DP71, Olympus) mounted on a BX51 Olympus microscope, and analyzed using a semi- automated macro built in Fiji (Schindelin et al., 2012) (v. 1.51) (Karlsen et al., 2019), for muscle fiber cross- sectional area. .. Cells were fixed using Histofix (Histolab), and then stained for ChAT (AB144P; Sigma- Aldrich) and Tau1 (GTX130462; GeneTex) with appropriate fluorescent secondary antibodies (A11057, Invitrogen, and 711–545- 152, Jackson ImmunoResearch Laboratories). .. Coverslips were mounted on slides with mounting medium (P36931; Thermo Fisher Scientific) containing DAPI.



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    Chr4 Δ70/Δ70 does not affect pancreatic β-cell number but may reduce the cell proliferation rate already in young Ldlr −/− ApoB 100/100 mice. ( A ) Pancreatic islet size, ( B ) proportion of α- and β-cells, and ( C ) proliferation of mixed and ( D ) >10,000 μm 2 pancreatic islets in young Ldlr −/− ApoB 100/100 ( n = 5–7) and Chr4 Δ70/Δ70 mice ( n = 5–7). Primary antibody against glucagon was used as a marker for the pancreatic α-cells, and insulin ab was used for the β-cells. For visualization, <t>fluorescent</t> secondary antibodies were used. In the representative figures, glucagon positive cells appear in red and insulin in green. Nuclei were counterstained with DAPI (blue). Ki-67 antibody was used as a marker of cell proliferation and visualized with DAB. ( E ) For the islet function, insulin secretion of Ldlr −/− ApoB 100/100 ( n = 4) and Chr4 Δ70/Δ70 mice ( n = 4) was measured both in fasted state (0 min) and in response to 1 g/kg i.p. glucose at time points 15, 30, 60 and 90 min after the administration. Asterisk (*) indicates statistical significance. Difference in mean between Ldlr −/− ApoB 100/100 and Chr4 Δ70/Δ70 mice was measured by using t -test, and it was considered statistically significant when p < 0.05.
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    Chr4 Δ70/Δ70 does not affect pancreatic β-cell number but may reduce the cell proliferation rate already in young Ldlr −/− ApoB 100/100 mice. ( A ) Pancreatic islet size, ( B ) proportion of α- and β-cells, and ( C ) proliferation of mixed and ( D ) >10,000 μm 2 pancreatic islets in young Ldlr −/− ApoB 100/100 ( n = 5–7) and Chr4 Δ70/Δ70 mice ( n = 5–7). Primary antibody against glucagon was used as a marker for the pancreatic α-cells, and insulin ab was used for the β-cells. For visualization, <t>fluorescent</t> secondary antibodies were used. In the representative figures, glucagon positive cells appear in red and insulin in green. Nuclei were counterstained with DAPI (blue). Ki-67 antibody was used as a marker of cell proliferation and visualized with DAB. ( E ) For the islet function, insulin secretion of Ldlr −/− ApoB 100/100 ( n = 4) and Chr4 Δ70/Δ70 mice ( n = 4) was measured both in fasted state (0 min) and in response to 1 g/kg i.p. glucose at time points 15, 30, 60 and 90 min after the administration. Asterisk (*) indicates statistical significance. Difference in mean between Ldlr −/− ApoB 100/100 and Chr4 Δ70/Δ70 mice was measured by using t -test, and it was considered statistically significant when p < 0.05.
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    anti appropriate fluorescent secondary  (Cell Signaling Technology Inc)
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    Chr4 Δ70/Δ70 does not affect pancreatic β-cell number but may reduce the cell proliferation rate already in young Ldlr −/− ApoB 100/100 mice. ( A ) Pancreatic islet size, ( B ) proportion of α- and β-cells, and ( C ) proliferation of mixed and ( D ) >10,000 μm 2 pancreatic islets in young Ldlr −/− ApoB 100/100 ( n = 5–7) and Chr4 Δ70/Δ70 mice ( n = 5–7). Primary antibody against glucagon was used as a marker for the pancreatic α-cells, and insulin ab was used for the β-cells. For visualization, <t>fluorescent</t> secondary antibodies were used. In the representative figures, glucagon positive cells appear in red and insulin in green. Nuclei were counterstained with DAPI (blue). Ki-67 antibody was used as a marker of cell proliferation and visualized with DAB. ( E ) For the islet function, insulin secretion of Ldlr −/− ApoB 100/100 ( n = 4) and Chr4 Δ70/Δ70 mice ( n = 4) was measured both in fasted state (0 min) and in response to 1 g/kg i.p. glucose at time points 15, 30, 60 and 90 min after the administration. Asterisk (*) indicates statistical significance. Difference in mean between Ldlr −/− ApoB 100/100 and Chr4 Δ70/Δ70 mice was measured by using t -test, and it was considered statistically significant when p < 0.05.
    Anti Appropriate Fluorescent Secondary, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Chr4 Δ70/Δ70 does not affect pancreatic β-cell number but may reduce the cell proliferation rate already in young Ldlr −/− ApoB 100/100 mice. ( A ) Pancreatic islet size, ( B ) proportion of α- and β-cells, and ( C ) proliferation of mixed and ( D ) >10,000 μm 2 pancreatic islets in young Ldlr −/− ApoB 100/100 ( n = 5–7) and Chr4 Δ70/Δ70 mice ( n = 5–7). Primary antibody against glucagon was used as a marker for the pancreatic α-cells, and insulin ab was used for the β-cells. For visualization, fluorescent secondary antibodies were used. In the representative figures, glucagon positive cells appear in red and insulin in green. Nuclei were counterstained with DAPI (blue). Ki-67 antibody was used as a marker of cell proliferation and visualized with DAB. ( E ) For the islet function, insulin secretion of Ldlr −/− ApoB 100/100 ( n = 4) and Chr4 Δ70/Δ70 mice ( n = 4) was measured both in fasted state (0 min) and in response to 1 g/kg i.p. glucose at time points 15, 30, 60 and 90 min after the administration. Asterisk (*) indicates statistical significance. Difference in mean between Ldlr −/− ApoB 100/100 and Chr4 Δ70/Δ70 mice was measured by using t -test, and it was considered statistically significant when p < 0.05.

    Journal: Cells

    Article Title: Deletion of the Murine Ortholog of the Human 9p21.3 Locus Leads to Insulin Resistance and Obesity in Hypercholesterolemic Mice

    doi: 10.3390/cells13110983

    Figure Lengend Snippet: Chr4 Δ70/Δ70 does not affect pancreatic β-cell number but may reduce the cell proliferation rate already in young Ldlr −/− ApoB 100/100 mice. ( A ) Pancreatic islet size, ( B ) proportion of α- and β-cells, and ( C ) proliferation of mixed and ( D ) >10,000 μm 2 pancreatic islets in young Ldlr −/− ApoB 100/100 ( n = 5–7) and Chr4 Δ70/Δ70 mice ( n = 5–7). Primary antibody against glucagon was used as a marker for the pancreatic α-cells, and insulin ab was used for the β-cells. For visualization, fluorescent secondary antibodies were used. In the representative figures, glucagon positive cells appear in red and insulin in green. Nuclei were counterstained with DAPI (blue). Ki-67 antibody was used as a marker of cell proliferation and visualized with DAB. ( E ) For the islet function, insulin secretion of Ldlr −/− ApoB 100/100 ( n = 4) and Chr4 Δ70/Δ70 mice ( n = 4) was measured both in fasted state (0 min) and in response to 1 g/kg i.p. glucose at time points 15, 30, 60 and 90 min after the administration. Asterisk (*) indicates statistical significance. Difference in mean between Ldlr −/− ApoB 100/100 and Chr4 Δ70/Δ70 mice was measured by using t -test, and it was considered statistically significant when p < 0.05.

    Article Snippet: Pancreatic α- and β-cells were immunostained with primary antibodies against glucagon (Dako A0565, Rabbit anti-human glucagon, Agilent, Santa Clara, CA, USA) and insulin (Dako A0564, Guinea pig anti-insulin, Agilent, Santa Clara, CA, USA) and appropriate fluorescent secondary antibodies (for glucagon, A21442, chicken anti-rabbit A594, Thermo Fisher Scientific, Waltham, MA, USA, and for insulin, BA-7000 Goat anti-guinea pig with A-2011 Fluorescein Avidin DCS, Vector laboratories, Newark, CA, USA).

    Techniques: Marker